HPLC同时测定乳癖消颗粒中三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1的含量

Determination of Notoginsenoside R1, Ginsenoside Rg1 and Ginsenoside Rb1 in Rupixiao Granule by HPLC

关键词:  高效液相色谱法  乳癖消颗粒  三七皂苷R1  人参皂苷Rg1  人参皂苷Rb1

Key words:  HPLC  Rupixiao granule  notoginsenoside R1  ginsenoside Rg1  ginsenoside Rb1

摘要:

目的 建立高效液相色谱法测定乳癖消颗粒中三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1含量。 方法 色谱柱为CAPCELL PAK C₁₈(150 mm×4.6 mm，5 μm)；流动相A为乙腈，B为水；流速：1.0 mL·min^-1；检测波长为203 nm；洗脱程序：0~12 min，A相19%，B相81%；12~60 min，A为19%→36%，B为81%→64%。结果 三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1保留时间分别为21，25，51 min，与各自相邻峰的分离度均在1.5以上。三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1的线性范围分别为0.100 2~2.00 4 μg、0.418 8~8.376 μg和0.187~3.74 μg。三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1的回收率分别为98.5%，99.6%和96.5%，RSD分别为1.9%，1.3%和1.9%。结论 本法简便、准确、重现性好，可用于乳癖消颗粒中三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1 含量的同时测定。

Abstract:

OBJECTIVE To establish an HPLC method for determination of notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 in Rupixiao granule. METHODS The chromatographic conditions included C18 column(CAPCELL PAK, 150 mm×4.6 mm, 5 μm), acetonitrile as mobile phase A, water as mobile phase B. The flow rate was 1.0 mL·min^-1. The detection wavelength was 203 nm. Gradient elution: 0-12 min, A: 19%, B: 81%; 12-60 min, A:19%→36%, B: 81→64%. RESULES The retention time of notoginsenoside R1, Ginsenoside Rg1 and Ginsenoside Rb1 was 21, 25, 51 min, respectively. The resolution was above 1.5. The linear range of notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 was 0.100 2-2.004 μg, 0.418 8-8.376 μg and 0.187-3.74 μg. The average recovery was 98.5%, 99.6% and 96.5%, RSD was 1.9%,1.3% and 1.9%, respectively. CONCLUSION The method is simple, accurate and reproduciable. It can be used for routine analysis of notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 in Rupixiao granule simultaneously.